椪柑萎缩型枯水病和粒化型枯水病几种生理指标差异的研究

分析了椪柑萎缩型枯水与粒化型枯水以及正常果的生理差异。发现萎缩型枯水果的失重、呼吸速率显著高于粒化型枯水果,但其果肉水势和出汁率显著低于粒化型枯水果和正常果;萎缩型枯水与粒化型枯水一样,其脱落酸大量积累,14C-葡萄糖大量转化为不溶性物质。认为萎缩型枯水机理在于椪柑果实大量失水引发了逆境生理代谢,导致大量可溶性营养消耗,汁胞萎缩崩溃。     

改良背主动脉注射法对鸡胚发育与转基因表达效率的影响

背主动脉注射是生产转基因鸡的经典方法,该方法不需换壳培养,但壳内注射技术难度大,并且无法实时观察后期胚胎发育情况。本实验对背主动脉注射法进行了改进,将增强型绿色荧光蛋白(EGFP)腺相关病毒(Adeno-associated Virus,AAV)壳外注射至150枚HH 14~16期(Hamburger-Hamilton Stage 14~16)鸡胚背主动脉中,再进行换壳培养,继续孵化至出壳,观察和分析改良背主动脉注射法与传统背主动脉注射法和胚盘下腔注射法对发育8、14、18、21 d鸡胚存活率、孵化率以及EGFP阳性检出率的影响。结果表明:改良背主动脉注射组鸡胚存活率均高于传统背主动脉注射组与胚盘下腔注射组(P<0.05或P<0.01);改良背主动脉注射组的鸡胚孵化率(37%)高于传统背主动脉注射组(16%)与胚盘下腔注射组(28%)(P<0.01);荧光蛋白手电筒检测显示,改良背主动脉注射组鸡胚EGFP阳性率(17%)明显高于传统背主动脉注射组(13%)与胚盘下腔注射组(12%)(P<0.01)。综上,背主动脉壳外注射结合换壳培养提高了转基因鸡胚胎孵化率和EGFP阳性检出率,对提高转基因鸡效率具有重要价值。     

System Design for Leather Cavity XCT

This paper introduces a method of solving the problem of leather cavity measurement in shoe CAD/CAM by industrial CT technology. Concentrating on peculiarity and requirement of leather cavity measurement, this paper presents a X-ray industrial CT system scheme, which not only satisfy the requirement of leather cavity measurement but also can achieve general industrial CT. measurement. The achievement on data collection system and motion control of stepping motor is emphasized.     

Penetration behavior of bath induced by top-blown gas jets for steelmaking converter

The cavity induced by top-blow gas jets impinging on the bath surface is one of most important parameters, which has overwhelming influence on the rate of slag formation and metallurgical performance. Water model experiments are conducted through a 1/10 scaled-down top-blown convertor. Effects of lance height and gas flow rate on penetration behavior are studied, and also the influence mechanism of liquid surface tension on penetration depth is addressed based on the experiments and theory analysis. An energy utilization index (EUI) is defined to reflect how much the impinging kinetic energy of gas jets are transferred into bath for formation of cavity, and an improved model is proposed to predict penetration depth of jets. The results show that penetration depth increases with lowering lance height and increasing gas flow rate, and impact diameter increases with increasing lance height, but is little affected by gas flow rate. Influence of liquid surface tension on penetration depth is enhanced with increasing surface tension and penetration depth. The EUI increases with increasing lance height, and a function relationship of it with lance height is obtained based on the experiments.     

Cavity spot of carrots ‐ an association with soil ammonium

Abstract

Cavity spot incidence on sixteen samples of carrots from four fields was found to be correlated with soil ammonium (r = + .78***) and pH (r = ‐ .63**). The calcium concentration in the carrots related to soil ammonium (r = ‐ .65**), but not to cavity spot incidence. Soil nitrate and ammonium were positively associated with each other (r = + .63**), and their ratio (NH₄/NO₃) was very highly correlated with exchangeable manganese (r = + .93***) suggesting that anaerobicity was present.     

不同腔长下调Q激光器输出脉冲特性研究

从Nd3+:YAG脉冲激光器调Q技术出发,研究了不同腔长条件下电光调Q激光器输出脉冲的宽度、能量和峰值功率特征,得到了不同腔长的调Q激光器输出脉冲特性曲线。研究结果表明,激光器腔长的改变能较好地调节脉冲的输出特性,对脉冲激光器的灵活应用具有一定的指导意义。     

Assessment of the response of carrot somaclones to Pythium violae, causal agent of cavity spot

Cavity spot is a major disease of carrots, causing cavities on the surface of the root. Available commercial varieties show a range of susceptibility but no significant resistance. Seed progeny from 46 tissue culture-derived carrot ( Daucus carota ) somaclones were screened for viability, then 19 selected somaclone families were sown under glasshouse conditions, along with commercial cultivars (Bertan, Nandor, Bolero and Vita Longa) as controls. Mature roots were exposed to Pythium violae in a cavity spot bioassay to determine their response as measured by disease incidence and severity. Some somaclones formed fewer lesions than the least susceptible control cultivar, Vita Longa. Seven somaclonal families that showed a range of susceptibility were sown under field conditions and the assessment was repeated. Although there was little relationship between glasshouse and field-trial results, under field conditions one of the somaclones had a mean incidence of disease, as estimated by transformed data, of 1·9 compared with 37·9 for the most susceptible somaclone and 3·5 for Bolero, the most resistant commercial cultivar. The results indicated that significant genetic variation in susceptibility to cavity spot disease was present in the somaclones.     

犬声带切除术的研究

犬声带切除术是目前小动物临床外科的常见手术，主要目的是消除或降低犬的吠叫声音。切除声带的手术途径有两种，即经口腔切除和经腹侧喉室切除。选取20只2岁左右的杂种犬，随机分成2组。第l组采用腹侧喉室声带切除术，术后半年的消声率为80oA；第2组采用经口腔声带切除术，术后半年的消声率为40％。但采用经口腔声带切除术，手术比较简便和快捷，损伤小，恢复较快。因此，经口腔声带切除术是一种较好的犬声带切除方法。     

一种水窖入水口的自闭系统初探

在对水窖较为普遍的窖体破坏、塌窖等原因进行分析的基础上,引入一种防止水窖超蓄塌陷的水窖入水口自闭系统.此系统结构简单、造价低、使用方便,能及时自闭入水口且关闭效果好,保证水窖蓄水不超过限制水位,从而保障水窖的结构稳定,延长水窖的使用年限.